The Bioparque Pantanal in Campo Grande, Brazil, has reached a significant milestone by successfully reproducing 100 different aquatic species in a controlled environment, positioning it as the world’s largest living genetic bank for freshwater organisms. This achievement accounts for over 21% of the total number of animals at the Bioparque, highlighting its crucial role in conservation efforts. Among the 100 species bred at the Bioparque, over 4,000 individuals were born there, with 29 representing new records globally and 20 being first-time records for Brazil. Notably, three of these species are classified as endangered. The diversity of species reflects the ecological richness of Brazil, with representations from various biomes including the Pantanal, Amazon, Cerrado, Atlantic Forest, and Caatinga, in addition to species from other continents. The aquarium showcases rare and endangered species such as the Cascudo-viola, which carries its eggs in its mouth until they hatch, and the Mexican axolotl, known for its remarkable tissue regeneration abilities. This impressive collection emphasizes the importance of biodiversity and conservation in aquatic ecosystems, underlining the Bioparque’s commitment to protecting these vital species and educating the public about their importance and vulnerability.